Metabolomics Studies on Yeast Arginine Synthesis Pathway Mutants Using maXis impact with ionBooster
نویسندگان
چکیده
Polar metabolites of the arginine biosynthetic pathway are poorly retained in reversed phase C18 chromatography. Hydrophilic interaction chromatography (HILIC) is an option for separating polar metabolites such as amino acids. However, compared to RP chromatography, peaks in HILIC separations are often broader and column re-equilibration requires a longer time. To enable metabolic profiling of ionic and polar metabolites using standard C18 chromatographic separation, the extracted polar metabolites were dansylated according to a protocol published by Guo and Li [1].
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